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mapseq sindbis viral barcode library  (Cold Spring Harbor Laboratory Meetings)

 
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    Structured Review

    Cold Spring Harbor Laboratory Meetings mapseq sindbis viral barcode library
    ( A ) Brightfield and epifluorescence microscope images of the injection site on a sagittal brain section showing the spread of neurons infected by the <t>MAPseq</t> virus library in wild-type mice. ( B ) A neuron’s position in the dorso-ventral axis does not affect the likelihood of collateralization of its axons to the hippocampus (paired two-tailed Wilcoxon signed rank test, p = 1, median proportion of collateralizing barcodes in dEC = 99.3%, vEC = 99.8%, n = 3 mice).
    Mapseq Sindbis Viral Barcode Library, supplied by Cold Spring Harbor Laboratory Meetings, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mapseq+sindbis+viral+barcode+library/pmc08940174-158-15-30?v=Cold+Spring+Harbor+Laboratory+Meetings
    Average 90 stars, based on 1 article reviews
    mapseq sindbis viral barcode library - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Telencephalic outputs from the medial entorhinal cortex are copied directly to the hippocampus"

    Article Title: Telencephalic outputs from the medial entorhinal cortex are copied directly to the hippocampus

    Journal: eLife

    doi: 10.7554/eLife.73162

    ( A ) Brightfield and epifluorescence microscope images of the injection site on a sagittal brain section showing the spread of neurons infected by the MAPseq virus library in wild-type mice. ( B ) A neuron’s position in the dorso-ventral axis does not affect the likelihood of collateralization of its axons to the hippocampus (paired two-tailed Wilcoxon signed rank test, p = 1, median proportion of collateralizing barcodes in dEC = 99.3%, vEC = 99.8%, n = 3 mice).
    Figure Legend Snippet: ( A ) Brightfield and epifluorescence microscope images of the injection site on a sagittal brain section showing the spread of neurons infected by the MAPseq virus library in wild-type mice. ( B ) A neuron’s position in the dorso-ventral axis does not affect the likelihood of collateralization of its axons to the hippocampus (paired two-tailed Wilcoxon signed rank test, p = 1, median proportion of collateralizing barcodes in dEC = 99.3%, vEC = 99.8%, n = 3 mice).

    Techniques Used: Microscopy, Injection, Infection, Virus, Two Tailed Test

    ( A ) Experimental strategy. A MAPseq virus library was injected into deep medial entorhinal cortex (MEC; n = 3 mice). Forty-four hours later, mice were sacrificed and brains were serially sectioned in the sagittal plane at 400 μm thickness. Tissue from five major divisions (isocortex, CNU, Olf/Ctxsp, dHip, and vHip), as well as dorsal and ventral MEC were then dissected and collected in tubes. Brainstem and spinal cord tissue was collected as a negative control. Tissue was further processed for RNA extraction and next generation sequencing. ( B ) Nearly all barcodes detected in CNU, Olf/Ctxsp, and isocortex were also detected in the hippocampus (isocortex: 98.6% ± 0.06%; CNU: 99.2% ± 0.08%; Olf/Ctxsp: 99.0% ± 0.4%; n = 3 mice, 1603, 3493, and 1677 barcodes from brains 1, 2, and 3, respectively). ( C ) Dorso-ventral topography of projections revealed by relative barcode counts. For each barcode, counts were normalized to show the relative projection strength of each neuron to dorsal and ventral hippocampus ( n = 3 mice).
    Figure Legend Snippet: ( A ) Experimental strategy. A MAPseq virus library was injected into deep medial entorhinal cortex (MEC; n = 3 mice). Forty-four hours later, mice were sacrificed and brains were serially sectioned in the sagittal plane at 400 μm thickness. Tissue from five major divisions (isocortex, CNU, Olf/Ctxsp, dHip, and vHip), as well as dorsal and ventral MEC were then dissected and collected in tubes. Brainstem and spinal cord tissue was collected as a negative control. Tissue was further processed for RNA extraction and next generation sequencing. ( B ) Nearly all barcodes detected in CNU, Olf/Ctxsp, and isocortex were also detected in the hippocampus (isocortex: 98.6% ± 0.06%; CNU: 99.2% ± 0.08%; Olf/Ctxsp: 99.0% ± 0.4%; n = 3 mice, 1603, 3493, and 1677 barcodes from brains 1, 2, and 3, respectively). ( C ) Dorso-ventral topography of projections revealed by relative barcode counts. For each barcode, counts were normalized to show the relative projection strength of each neuron to dorsal and ventral hippocampus ( n = 3 mice).

    Techniques Used: Virus, Injection, Negative Control, RNA Extraction, Next-Generation Sequencing



    Similar Products

    mapseq sindbis viral barcode library  (Cold Spring Harbor Laboratory Meetings)
    90
    Cold Spring Harbor Laboratory Meetings mapseq sindbis viral barcode library
    ( A ) Brightfield and epifluorescence microscope images of the injection site on a sagittal brain section showing the spread of neurons infected by the <t>MAPseq</t> virus library in wild-type mice. ( B ) A neuron’s position in the dorso-ventral axis does not affect the likelihood of collateralization of its axons to the hippocampus (paired two-tailed Wilcoxon signed rank test, p = 1, median proportion of collateralizing barcodes in dEC = 99.3%, vEC = 99.8%, n = 3 mice).
    Mapseq Sindbis Viral Barcode Library, supplied by Cold Spring Harbor Laboratory Meetings, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mapseq+sindbis+viral+barcode+library/pmc08940174-158-15-30?v=Cold+Spring+Harbor+Laboratory+Meetings
    Average 90 stars, based on 1 article reviews
    mapseq sindbis viral barcode library - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Brightfield and epifluorescence microscope images of the injection site on a sagittal brain section showing the spread of neurons infected by the MAPseq virus library in wild-type mice. ( B ) A neuron’s position in the dorso-ventral axis does not affect the likelihood of collateralization of its axons to the hippocampus (paired two-tailed Wilcoxon signed rank test, p = 1, median proportion of collateralizing barcodes in dEC = 99.3%, vEC = 99.8%, n = 3 mice).

    Journal: eLife

    Article Title: Telencephalic outputs from the medial entorhinal cortex are copied directly to the hippocampus

    doi: 10.7554/eLife.73162

    Figure Lengend Snippet: ( A ) Brightfield and epifluorescence microscope images of the injection site on a sagittal brain section showing the spread of neurons infected by the MAPseq virus library in wild-type mice. ( B ) A neuron’s position in the dorso-ventral axis does not affect the likelihood of collateralization of its axons to the hippocampus (paired two-tailed Wilcoxon signed rank test, p = 1, median proportion of collateralizing barcodes in dEC = 99.3%, vEC = 99.8%, n = 3 mice).

    Article Snippet: 5 C57Bl6J adult male and female mice were injected in the deep MEC with the MAPseq Sindbis viral barcode library (3 × 10 10 GC/ml) provided by the MAPseq facility (Cold Spring Harbor Laboratories).

    Techniques: Microscopy, Injection, Infection, Virus, Two Tailed Test

    ( A ) Experimental strategy. A MAPseq virus library was injected into deep medial entorhinal cortex (MEC; n = 3 mice). Forty-four hours later, mice were sacrificed and brains were serially sectioned in the sagittal plane at 400 μm thickness. Tissue from five major divisions (isocortex, CNU, Olf/Ctxsp, dHip, and vHip), as well as dorsal and ventral MEC were then dissected and collected in tubes. Brainstem and spinal cord tissue was collected as a negative control. Tissue was further processed for RNA extraction and next generation sequencing. ( B ) Nearly all barcodes detected in CNU, Olf/Ctxsp, and isocortex were also detected in the hippocampus (isocortex: 98.6% ± 0.06%; CNU: 99.2% ± 0.08%; Olf/Ctxsp: 99.0% ± 0.4%; n = 3 mice, 1603, 3493, and 1677 barcodes from brains 1, 2, and 3, respectively). ( C ) Dorso-ventral topography of projections revealed by relative barcode counts. For each barcode, counts were normalized to show the relative projection strength of each neuron to dorsal and ventral hippocampus ( n = 3 mice).

    Journal: eLife

    Article Title: Telencephalic outputs from the medial entorhinal cortex are copied directly to the hippocampus

    doi: 10.7554/eLife.73162

    Figure Lengend Snippet: ( A ) Experimental strategy. A MAPseq virus library was injected into deep medial entorhinal cortex (MEC; n = 3 mice). Forty-four hours later, mice were sacrificed and brains were serially sectioned in the sagittal plane at 400 μm thickness. Tissue from five major divisions (isocortex, CNU, Olf/Ctxsp, dHip, and vHip), as well as dorsal and ventral MEC were then dissected and collected in tubes. Brainstem and spinal cord tissue was collected as a negative control. Tissue was further processed for RNA extraction and next generation sequencing. ( B ) Nearly all barcodes detected in CNU, Olf/Ctxsp, and isocortex were also detected in the hippocampus (isocortex: 98.6% ± 0.06%; CNU: 99.2% ± 0.08%; Olf/Ctxsp: 99.0% ± 0.4%; n = 3 mice, 1603, 3493, and 1677 barcodes from brains 1, 2, and 3, respectively). ( C ) Dorso-ventral topography of projections revealed by relative barcode counts. For each barcode, counts were normalized to show the relative projection strength of each neuron to dorsal and ventral hippocampus ( n = 3 mice).

    Article Snippet: 5 C57Bl6J adult male and female mice were injected in the deep MEC with the MAPseq Sindbis viral barcode library (3 × 10 10 GC/ml) provided by the MAPseq facility (Cold Spring Harbor Laboratories).

    Techniques: Virus, Injection, Negative Control, RNA Extraction, Next-Generation Sequencing